Symphytum officinale tincture free#

Interestingly, gene expression analysis for Runx-2 (n = 10), Osteopontin (n = 10), Osteocalcin (n = 10) and alkaline phosphatase activity (n = 8) also showed increased osteogenesis with the addition of Symphytum officinale to BDM, specially mother tincture. The efficiency of osteogenic differentiation (2 weeks) for different doses of Symphytum officinale was determined by flow cytometry (n = 10) for osteoblast marker, Osteocalcin, and most doses of Symphytum officinale enhanced osteogenesis. In folklore, Symphytum officinale roots were used in traditional medicine internally (as an herbal tea or tincture) or externally (as ointment, compresses, or alcoholic digestion clarification needed) for treatment of various disorders, including commonly as a treatment for reducing the pain of osteoarthritis. MTT assay showed that the selected doses of SO did not induce any cytotoxicity in MSCs (24 hours). Gene expression analyses for osteoblast markers (Runx-2, Osteopontin and Osteocalcin) were evaluated in differentiated osteoblasts using qPCR.įlow cytometry (CD90, CD105) detected MSCs isolated from bone marrow (93-98%). Different homoeopathic doses of SO (MT, 3C, 6C, 12C and 30C) were added to the basic differentiation medium (BDM) and efficiency of MSCs differentiating into osteoblasts were measured by evaluating expression of Osteocalcin using flow cytometry, and alkaline phosphatase activity using ELISA. Osteogenic differentiation was induced in MSCs with β-glycerophosphate, ascorbic acid and dexamethasone over 2 weeks. Cytotoxicity of SO was evaluated by MTT assay. MSCs were isolated, expanded and characterized by flow cytometry (CD90, CD105). This study aimed to determine if homoeopathic doses of SO could augment osteogenesis in MSCs as they differentiate into osteoblasts in vitro.īone marrow samples were obtained from patients who underwent bone grafting procedures (n = 15). Symphytum officinale (SO) is a medicinal plant and in homoeopathic literature, believed to accelerate bone healing. Buy your Baldwins Comfrey Leaf ( Symphytum Officinale ) Herbal Tincture from G Baldwin & Co one of the leading herbalists in London, supplying an extensive. Fresh organic leaf tincture - 1:5 25 - 500ml, 1000ml. Shop > Tinctures and fluid extracts > Symphytum officinale NB. Dosage-8 to 9 drops diluted in 2-3 teaspoon of normal water or as directed by your physician. This Handy Homeopathic kit contains two sealed Bottles of SBL Mother tincture 30ml each.

Symphytum officinale tincture free#

Mesenchymal stem cells (MSCs) are multipotent stem cells possessing regenerative potential. FREE MAINLAND SHIPPING ON ORDERS OVER £150 excluding VAT. Symphytum Off is indicated for the relief of swelling (inflammatory disorders such as bruises, sprains, pulled muscles or ligaments).